top of page

Epigenomics

The epigenome is a multitude of chemical compounds that can tell the genome what to do. 
 
 
Epigenetics focuses on processes that regulate how and when certain genes are turned on and turned off, while epigenomics pertains to analysis of epigenetic changes across many genes in a cell or entire organism.
 
The epigenomics changes are a combination of  DNA modifications and chromatin remodeling . 
 
FGC provides service for the analysis of chromatin remodeling: ATAC-seq and CHIP-seq. 
 
ATAC-seq - Assay for transposase-accessible chromatin sequencing (ATAC-seq).  This assay provides genome-wide profiles of open and accessible regions of chromatin that are indicative of active regulatory regions. The method probes DNA accessibility with hyperactive Tn5 transposase, which inserts sequencing adapters into accessible regions of chromatin. 
 
atac-seq.png

The mutant Tn5 transposases, preloaded with DNA adapters are inserted adapters into the DNA  of purified nuclei of the cells, tagging genomic DNA for downstream excision and fragmentation. The libraries are preparing fragmented form DNA following Illumina sequencing. One of the main advantages of ATAC-seq method is that a very low number of cells is necessary,  in a range of 500 to 50,000 cells. 

 

Our service: 

  •  Tagmentation and library preparation from freshly provided cells or from cells frozen by ATAC-seq compatible protocol ;

  • Sequencing of the libraries.

  • Bioinformatics analysis of the results. 

        Please contact FGC for the price and experimental design. 

 

 

CHIP-seq -  The combining chromatin immunoprecipitation (ChIP) assays with sequencing. ChIP-Seq identifies the binding sites of DNA-associated proteins and can be used to map global binding sites for a given protein. 

 

Our service(*): 

  • Library preparation from immunoprecipitated chromatin.

  • Sequencing of the libraries.

  • Bioinformatics analysis of the results

(*) Currently, FGC will not perform chromatin IP. We will provide the protocols and inquire core consultants in case additional support is needed. 

      Please contact FGC for the price and experimental design. 

bottom of page